ABSTRACT
ObjectiveTo investigate the polymorphisms of 124 individual identiifcation SNPs in Chinese Han using the Ion Personal Genome Machine?(PGMTM).Method Samples from 130 unrelated Chinese Han individuals and two families (8 genealogical individuals) were ampliifed using Ion AmpliseqTM Library kit and sequenced on Ion Torrent PGM? platform.Results 14 148 SNPs were detected.A total of 99.992 9% SNPs were correctly called by the HID SNP Genotyper v4.3 plugin, while 0.007 1% wrongly reported and 62 NN calls needed manual correction. The MP ranged from 0.348 0 (rs2831700) to 0.817 3 (rs740910) with the value of 6.898 4 × 10-34 for CMP. The DP ranged from 0.182 7 (rs740910) to 0.652 0 (rs1355366) with the value of 0.999 999 999 999 999 999 999 999 999 999 999 310 2 for CDP, which was larger than that of 22 STR loci. The PE ranged from 0.007 3 (rs1024116) to 0.278 1 (rs1058083) with the value of 0.999 999 616 7 for CPE, which was smaller than that of 22 STR loci. A total of 8 Y-SNP haplo-types were observed from 72 unrelated male samples. No mutation was observed from pedigrees.Conclusion The 124 IISNPs were high polymorphic in Chinese Han and they were ideal markers for human identiifcation. The PGMTM platform has a potential role in forensic science.
ABSTRACT
Saliva and samples containing saliva were regarded as a kind of potential biological trace evidencefor individual identification in forensic science practice. In this paper, the authors have studied theDNA analysis of saliva and saliva containing samples. The results demonstrated that DNA can be extracted from saliva and saliva-containing samples. RFLPs analysis by DNA fingerprinting and PCRbased typing can be performed according to the amount of DNA extracted from these samples.
ABSTRACT
The improvement of electrophoresis for DNA fingerprinting has been carried out. The time of electrophoresis was shortened,and 1/3-1/2 of reagents were saved. The minimal amount of DNA samples for DNA analysis was 0.8?g. It is worthy to popularize and apply in practice.
ABSTRACT
Objective To study the specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalian. Method The specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalians were amplified and directly se-quenced. The sequence data of PCR products of human beings and mammalians were declared. Results The sequences of 3'UTR of SON gene of human beings differed from 14 kinds of mammalians. The sequences of samples came from different human individuals did not show any difference. Conclusion The 3'UTR of SON gene is a good DNA region for specie identification. Using this technology, DNA of human beings can be distinguished from 14 kinds of mammalians. This technology is very useful in of forensic science.